Journal: iScience

Article Title: Long-term and potent IOP-lowering effect of IκBα-siRNA in a nonhuman primate model of chronic ocular hypertension

doi: 10.1016/j.isci.2022.104149

Figure Lengend Snippet:

Article Snippet: rabbit monoclonal anti-α-smooth muscle actin (α-SMA) , Bioss , Cat# bsm-33187M;RRID: AB_2910163.

Techniques: Recombinant, Software

Journal: Journal of Inflammation Research

Article Title: Development of Diagnostic and Predictive Models for COPD Based on Anoikis Resistance

doi: 10.2147/JIR.S534626

Figure Lengend Snippet: Pulmonary function parameters in mice were measured using the FM animal forced pulmonary function testing system. ( A ) PEF. ( B ) MMEF. ( C ) FEV25ms/FVC. ( D ) FEV50ms/FVC. ( E ) FEV75ms/FVC. ( F ) FEV100ms/FVC. ( G ) Representative images of H&E-stained lung tissue sections and IHC-stained sections for anti-human α-SMA (brown). ( H ) Mean linear intercepts (MLI) and mean alveolar number (MAN) in each group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: After blocking with 10% goat serum, the sections were incubated overnight at 4°C with an anti-α-SMA antibody (80008-1-RR, Proteintech).

Techniques: Staining

Journal: Cell Reports Medicine

Article Title: Injectable hydrogel with miR-222-engineered extracellular vesicles ameliorates myocardial ischemic reperfusion injury via mechanotransduction

doi: 10.1016/j.xcrm.2025.101987

Figure Lengend Snippet: GEL-TeEV patches ameliorated cardiac dysfunction in cardiac IRI remodeling (A) Establishment of IRI remodeling model after 30-min ischemia and then protected by GEL-TeEV patches followed by 3-week reperfusion in vivo . (B) Representative echocardiography images of sham, IRI+PBS, IRI+GEL, IRI+TeEV, and IRI+GEL-TeEV groups. (C) Left ventricle ejection fraction (EF). (D) Fractional shortening (FS). (E) Masson staining. Scale bar: 100 μm. (F) Cardiac fibrosis detected by Masson staining. (G) WB analysis of fibrosis-related protein (α-SMA). (H) Quantitative protein expression level of α-SMA. (I) Col1a1 gene expression level quantified by qPCR analysis. (J) Col3a1 gene expression level quantified by qPCR analysis. (K) Relative expression level of pathological cardiac genes, including ANP, BNP, and β-MHC. Data present mean ± SD; n = 6 per group. One-way ANOVA test followed by Bonferroni test was used for statistical analysis. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. See also Figure S5 .

Article Snippet: α -Smooth Muscle Actin (ACTA2) Rabbit mAb , ABclonal , Cat #A17910, RRID: AB_2861755.

Techniques: In Vivo, Staining, Expressing, Gene Expression

Journal: Cell Reports Medicine

Article Title: Injectable hydrogel with miR-222-engineered extracellular vesicles ameliorates myocardial ischemic reperfusion injury via mechanotransduction

doi: 10.1016/j.xcrm.2025.101987

Figure Lengend Snippet:

Article Snippet: α -Smooth Muscle Actin (ACTA2) Rabbit mAb , ABclonal , Cat #A17910, RRID: AB_2861755.

Techniques: Produced, Recombinant, Western Blot, Lysis, Bicinchoninic Acid Protein Assay, Staining, TUNEL Assay, CCK-8 Assay, cDNA Synthesis, Sequencing, Software

Journal: International journal of experimental pathology

Article Title: Vitronectin deficiency attenuates hepatic fibrosis in a non-alcoholic steatohepatitis-induced mouse model.

doi: 10.1111/iep.12306

Figure Lengend Snippet: FIGURE 5 Effects of VN deficiency on α-SMA expression in NASH livers. A-D, Images of enzymatic immunohistochemistry using anti-α- SMA antibody in the liver of WT (A,B) and VN−/− (C,D) mice fed with either MF (A,C) or CDAHFD (B,D) for 10 wks. Arrows: α-SMA positive cells. Scale bar: 50 μm. (E-H) Images of immunofluorescence staining using anti- α-SMA antibody in the liver of WT (E,F) and VN−/− (G,H) mice fed with either MF (E,G) or CDAHFD (F,H) for 10 wks. V: Vessel. Scale bar: 20 μm. (I,J) Western blot analysis of α-SMA in the liver of WT and VN−/− mice fed with MF and CDAHFD for 6 and 10 wks. (J) Relative expression levels of α-SMA protein in the livers. The raw values of the α-SMA protein levels were normalized against GAPDH protein levels, and the mean values were normalized against α-SMA protein levels in MF-fed WT mice for 6 wks. (K,L) Expression levels of α-Sma and Tgf-β1 mRNA in the livers of CDAHFD-fed VN−/− mice. The values represent means ± SEM of at least three mice per group at each specified week. *P < 0.05, **P < 0.01. #P < 0.05, ##P < 0.01, ###P < 0.001 vs MF. V, vascular lesion

Article Snippet: The primary antibodies in this study included the following: anti- VN (1:5000; a gift from Prof. Masao Hayashi, Ochanomizu University, Tokyo, Japan), anti- α- SMA (1:5000; ab7817, Abcam, Cambridge, UK) and anti- GAPDH (1:5000; ACR001PT, OriGene Technologies Inc., Rockville, MD).

Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Staining, Western Blot